REQUIRED: Bachelor’s degree in Chemistry, Biology, Physics, or related field.
Ability to work with complex laboratory apparati. Experience with Excel, Origin, or other spreadsheets. Knowledge of a wide variety of laboratory procedures including pipetting, making analytical standards, documenting procedures and results. Ability to help design scientific experiments and write procedures. Ability to effectively communicate and exchange information. Ability to work and communicate with specialists and non-specialists. Employ safe laboratory practices. Follow a written procedures precisely and ask questions when instructions are unclear. Understand and implement basic mass spectrometry theory and practices.
Working Conditions / Physical Demands
Ability to lift 20 pounds to mid-torso (for mounting Nanomate sprayer). Ability to stand for 2 hours at a time during sample preparation steps. Sufficient visual acuity to read 4 or 6 point font. Fluency in English including complex analytical chemistry and biochemistry terms.
• Thermo Orbitrap XL ESI mass spectrometer
• Advion Triversa Nanomate sample inlet system
• Agilent 7890B/G7250 GC-EI-QTOF
• Agilent 7890B/5977 GC-EI-MS
• Agilent 6890N/5973 GC-EI-MS
• Agilent 1200/6130 HPLC-MS
• Dionex Ultimate 3000 dual HPLC
• Sciex 4000 QTRAP triple quadrupole tandem MS
• Speed vac evaporator
• Nitrogen evaporator
The Associate Mass Spectroscopist’s (AMS) primary role will revolve around collecting mass spectra from polar and nonpolar metabolite extracts from five model organisms that have been exposed to various chemicals to help establish the toxicity of said chemicals. The AMS will maintain the Orbitrap mass spectrometer, record data from metabolite samples, process the data into the format needed, perform various quality control assessments, assist in method development, and help create any training documents needed to continue the project. The incumbent will need to be comfortable using a Thermo Orbitrap XL high resolution mass spectrometer, an Advion Triversa Nanomate infusion apparatus, and an Agilent 7890B/G7250 GC-EI-QTOF mass spectrometer.
The secondary roles of the AMS will be in support of research activities related to small molecule analysis and in the operation of the IU Mass Spectrometry Facility. Duties for these roles include preparing metabolite extracts from Drosophila and other eukaryotes, derivatizing those extracts for GC-MS analyses, processing the resulting data for collaborators, recording accurate mass spectra of novel compounds for MSF customers, assisting MSF personnel in instrument maintenance and performance verification, updating training documents, and training new users on walk-up MSF equipment.
Note: This position is grant funded with expected annual renewals. Renewals are contingent on the availability of grant funds.