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General Dynamics Information Technology

Biological Technician III - Lab Manager (Flow Cytometry Center)

Post Date:Feb 01, 2020Type:Full-Time
Start Date:2/1/2020Salary:
Location:US-MD-Silver Spring Job Reference:G2019-67466
Skills:
 

Job Description:

GDIT is seeking a Biological Technician III (Lab Manager) to support WRAIR's Flow Cytometry Center. The contractor shall

work directly with the lab PI (Chief Flow Cytometry Center and Malaria Vaccine Branch) and/or perform immunological testing of animal and human samples. Specifically, the contractor shall:

  1. Perform cellular assays such as flow cytometric assays, EliSpots, cytokine arrays, and assays that are currently under development in the laboratory) on an as needed basis;
  2. Perform functional assays (such as phagocytosis, ILSDA, motility assays, and any assay that is currently under development in the laboratory) to assess the biological activity of antibodies on an as needed basis.
  3. Identify, adapt, and standardize methods for measuring humoral and cellular immune responses to malaria and malaria vaccines in an effort to identify immune correlates of protection.
  4. Identify, with the guidance by MMRP investigators, the need for new, improved readout methods;
  5. Determine whether a “de novo” development of the assay is needed (especially new flow cytometric panels characterizing lymphocyte subsets that may play a crucial role) or a suitable protocol, that is adaptable to the FCC, is available in the literature;
  6. Maintain a current knowledge of novel readout methods by regularly attending seminars and conferences;
  7. Gain the skill of novel readout methods by taking courses and/or attending workshops that provide training on such methods;
  8. Perform rigorous testing of the new method and establish Standard Operating Procedures (SOP's) to insure that quality of work is the highest caliber;
  9. Write and communicate research papers and review articles in scientific journals and present results and concepts at meetings/conferences;
  10. Maintain detailed records on experimentation and sample inventories (printed and computer files);
  11. Maintain the equipment of the FCC on a regular basis (once a month), perform daily quality control measurements and initiate corrective actions in case of instrument failures;
  12. Teach other investigators using the FCC equipment as well as assist in the teaching of institute wide courses (such as the OCID, DLI etc.);
  13. Assist during CHMI trials with assays that measure parasitemia in study subjects (such as reading of Giemsastained blood smears); and
  14. Maintain and operate Government owned equipment (ELISA plate washers and readers, BIAcore affinity measurement equipment (such as the Attana), flow cytometers, cell counters, cell sorters, array reader, microfluidizer, fermentor, centrifuges, imaging systems, microscopes).
  15. Perform data analysis when instructed by the principal investigator.

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BS in a life sciences discipline

MS preferred

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2+

  1. Ability to work on clinical samples for immunological evaluation; proficient in animal work (any manipulation related with immunization, malaria challenge); Hybridoma production for monoclonal antibodies; proficient in parasite culture techniques and parasitological readout methods. Knowledge of laboratory management, supplies ordering/procurement with training and use of the Defense Medical Logistics Standard Support (DMLSS), Electronic Centralized Acquisition Tool (ECAT), and Medical Master Catalog (MMC) systems, Design and validation parameters for ELISA assay development and testing.
  2. Certified malaria microscopist and SharePoint administrator desired
  3. Desired training at Becton Dickinson and Miltenyi Biotec facilities for QC/QA of flow cytometers, Mesoscale array reader training by Mesoscale Diagnostics.

 

' We are GDIT. The people supporting some of the most complex government, defense, and intelligence projects across the country. We deliver. Bringing the expertise needed to understand and advance critical missions. We transform. Shifting the ways clients invest in, integrate, and innovate technology solutions. We ensure today is safe and tomorrow is smarter. We are there. On the ground, beside our clients, in the lab, and everywhere in between. Offering the technology transformations, strategy, and mission services needed to get the job done.

GDIT is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or veteran status, or any other protected class.





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General Dynamics Information Technology



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